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The mechanisms underlying the anti-inflammatory effects of IGF-1 remain incompletely understood, but IGF-1 appears to curb inflammation by skewing macrophages toward an M2 phenotype and increasing IL-10 and transforming growth factor- (TGF-) production (71)

Reducing that inflammation while stimulating collagen production produces a dual benefit that microneedling alone cannot achieve

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Antibodies Primary antibodies used in this study are as follows: NW6 rabbit anti-DP directed against the C-terminal domain of DP (64), DP2.15 mouse antibody directed against DPI and DPII (Thermo Fisher Scientific), DP2.17 mouse antibody directed against DPI (Thermo Fisher Scientific), 115F mouse monoclonal antibody directed against DP (generous gift of David Garrod (65)) or purified from hybridoma culture (MilliporeSigma), 1,407 chicken anti-Pg (Aves Laboratories), mouse anti-N-cadherin (Invitrogen), sheep anti-N-cadherin (R&D Systems), mouse anti--catenin (1G5