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Subsequently, biodistribution studies of DSePA were carried out in a mice model, which indicated that DSePA was maximally absorbed in the lung tissues [90]

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26S proteasome activity assay Briefly, pulverized liver samples (20 mg aliquot) were homogenized in 26 S proteasome lysis buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM magnesium chloride (MgCl 2) [pH 7.5]) with a hand-held Potter-Elvehjem homogenizer

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