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Similarly, in severe TBI patients, exposure to hyperbaric O 2 at 1.5 ATA for 60 min showed no increase in the CSF F2-isoprostane levels, microdialysate glycerol, and BAL inflammatory markers (IL-6 and IL-8), which were used to monitor potential O 2 toxicity after exposure to HBOT (Rockswold et al., 2010)

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The same group showed that measurements of total NAD + and NADH from homogenates of isolated mitochondria did not yield accurate measurements of the free levels ( + ]/[NADH] was reduced by DR ( Opposite to the fasting and DR-induced reductive shifts in the rodent mitochondrial (free) [NAD + ]/[NADH], isolated mouse liver total mitochondrial NAD + /NADH showed an oxidative shift with fasting (Nakagawa et al., 2009)