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Prior to the experiment, the experimental box should be thoroughly cleaned and free of odors, with particular attention paid to removing any feces and urine from previous tests at the bottom of the box

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Likewise, senescent MSTN-deficient mice were shown to display improved fractional shortening, smaller left ventricular diastolic and systolic diameters, and decreased cardiac fibrosis 80 , although an earlier study reported that MSTN has no significant effect on cardiac hypertrophy or fibrosis 81