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Vitamin B12 increased methylation levels of CpGs in regulatory regions of CCL3 The effects of COVID-19 and B12 on the methylation levels of the 21 CpGs located in the promoter region and proximal portion of the first exon of the CCL3 gene (GRCh38/hg38 chr17: 36,090,276 - 36,090,005) were evaluated by Bisulfite Sequencing PCR in an NGS platform from DNA libraries produced with aliquots of endpoints A and B from individuals representing MOD, SEV and CTRL groups

In some ways, the lesser degree of obesity and exacerbated metabolic disturbances in these mice resembles the clinical characteristics of Asian patients with T2DM 17,18 , suggesting that Insr P1195L/ +/HFD mice are suitable for investigating the pathophysiology of HFD-sensitive T2DM in humans