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To visualize small-molecule metabolites localized within TLS, we used matrix-assisted laser desorption/ionization time of flight imaging mass spectrometry and found significant accumulation of glutathione (reduced form: glutathione [GSH], oxidized form: glutathione disulfide [GSSG]) within TLS (Figure 1C)

Recombinant Bet v 1 Plasmid vector pET-44 EK/LIC including Bet v 1 sequence (UniProtKB P43179), previously constructed in our laboratory, was transformed into ClearColi BL21 (DE3) Electrocompetent Cells (#60810-1, Lucigen, USA), the competent cells with a modified LPS that does not trigger the endotoxic response

coli supplemented with glucose also led to an increase in the AGE carboxymethyl-lysine (CML) as detected by ELISA (Figure 1c)

A recent report revealed that ferroptosis inducers, such as RSL3 and erastin, induced the ASK1-p38 MAPK pathway activation downstream of lipid ROS in A549 cells [43]