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To determine the sample size a power calculation was performed

What I find very, very disturbing is that the representative mentioned the goal is to change peoples behavior so they dont get addicted to nicotine

All procedures and the experimental design were approved by the Ethical Committee for Animal Research of the Federal University of Ouro Preto (Protocol n 2015/35), according to the Brazilian Society of Neuroscience and Behaviour and the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals (8th edition, 2011)

[2] Once in the bloodstream, nicotine circulates throughout the body and crosses the blood-brain barrier to bind with nicotinic acetylcholine receptors