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Nat Rev Neurol 16:303318 Tonda-Turo C, Origlia N, Mattu C, Accorroni A, Chiono V (2018) Current limitations in the treatment of Parkinsons and Alzheimers diseases: state-of-the-art and future perspective of polymeric carriers

doi: 10.1016/S0021-9258(19)36991-1 91 SudhaharVOkurMNBagiZO'BryanJPHayNMakinoAet al

For sensory neuron differentiation (46), iPSCs were cultured to 60%70% confluence, dissociated with accutase for 5 minutes, pelleted at 250 g for 3 minutes, plated on Matrigel-coated plates at 26,000 cells/cm 2 , and cultured in E8 medium with 5 M ROCK inhibitor for 24 hours

There is current debate about this which will be studied further