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Description

The activity was followed by measuring the quantity of radioactive diol formed in the aqueous phase using a scintillation counter (TriCarb 2810 TR, Perkin Elmer, Shelton, CT)

Following fixation with 4% paraformaldehyde for 30-60 min and nuclear staining with 0.02% DAPI (Sigma-Aldrich, #D9542) for 10 min, slices were scanned in a 20-fold magnification by using a Zeiss Axiovert 200M microscope equipped with Hamamatsu Orca Flash 4.0 camera and TissueFAXS scanning software (TissueGnostics, Vienna, Austria)