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Interventions such as caffeine supplementation have shown promise in complementing antioxidant therapy by preserving BBB integrity and mitigating inflammatory responses in the CNS [63,258]

DNA methylation typically occurs at CpG sites, where cytosine in the CpG dinucleotide environment is methylated at the fifth carbon position by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5 mC) (Figure 1)

All reagents and experiments conducted in this study are compliant with the institutional biological safety committee (SPROTO202200000081) of the University of Arkansas for Medical Sciences

If CrAT can generate acetyl-CoA for histone acetylation, we anticipated that the supplementation of acetylcarnitine to the culture media should boost histone acetylation and proliferation in lipid-depleted DKO cells, and this was indeed the case (Figure 6B, C)