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Additionally, these methods require highly specialised equipment and extensive lab-based technical support, resulting in high costs and limiting their applications in resource-limited laboratories and medical institutions

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Also it occurs at an activated carbon atom, that is next to sp , sp2 carbons: Oxidative Phase-I involving cytochrome P-450 enzymes: Aliphatic hydroxylation: Mainly occur on the ultimate () or penultimate (-1) carbon atom in the structure