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BD Biosciences, San Jose, CA, USA) according to the manufacturers instructions

The Spearman correlation between quantification by the two MS approaches was elevated (r = 0.953, P = 0.0001)

Results By comparing the activation of both systems in fission yeast, and applying mathematical equations to the experimental data, we estimate the toxic threshold of intracellular H 2 O 2 able to halt aerobic growth, and the temporal gradients of extracellular to intracellular peroxides