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The duplexed region can be connected by a linker, e.g., a nucleotide linker, e.g., 3, 4, 5, or 6 dTs, e.g., modified dTs

Reactions were performed with the qScript One-Step SYBR Green RT-qPCR, Low ROX Kit (Quantabio, Beverly, MA) in an AriaMx Real-time PCR System (Agilent, Savage, MD)

CD8+ T cell clones were pre-stained with 0.5 M CellTraceTM Violet (CTV) (Life TechnologiesTM) following manufacturer's protocols prior to seeding into APC wells

309 Here, we summarize preclinical and human studies revealing the implications and mechanisms of various dietary patterns on other diseases in addition to cancer, including neurodegenerative diseases, autoimmune diseases, CVD, and metabolic disorders