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For example, the NPs may build up on cell surfaces (Zhu et al., 2009

Biol Reprod (2000) 63(5):128593

Holmstrom and Finkel, 2014)

Following this, 0.1 ml of the supernatant was added to 0.5 ml of scintillation fluid (Ultima Gold, Perkin Elmer) and the radioactivity was estimated on MicroBeta scintillation counter (Perkin Elmer)