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Proteins of interest were probed with primary antibodies against GS (Millipore, MAB302), YAP (Cell Signaling Technology, D8H1X), phospho-YAP (Ser127, Cell Signaling Technology, D9W2I), -tubulin (Sigma-Aldrich, T5168), GAPDH (Santa Cruz Biotechnology, SC365062), Lamin B1 (Santa Cruz Biotechnology, SC377000), LATS1 (Cell Signaling Technology, C66B5), phospho-LATS1 (Ser909, Cell Signaling Technology, 9157), MST1 (Cell Signaling Technology, 3682), phospho-MST1/2 (Cell Signaling Technology, E7U1D), then probed with horseradish peroxidaseconjugated secondary antibodies (GE Healthcare) and visualized by ECL (SuperSignal West Dura Extended Duration Substrate, Thermo Fisher Scientific)

Cancer 9, 37

Shamsad A, Gautam T, Singh R, Banerjee M

Our in vivo findings provide evidence that the glutathione metabolism pathway contributes to ESCC progression via inhibition of the NF-kB signaling pathway and provides a groundwork for further development of a glutathione metabolism inhibitor to treat ESCC