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The transient methods, necessary to survey the large number of mutants in this project, introduce considerable measurement noise originating with variable level expression and brightly fluorescent dead cells

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In parallel, excessive Cu + generated in GSH-rich reductive environments can bind lipoylated mitochondrial TCA-cycle proteins such as DLAT, inducing aberrant protein aggregation and triggering cuproptosis across multiple cancer types 187,189,192-194