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Because BCAAs are converted to CoA compounds and used for energy generation via the Krebs cycle, they are implicated in mitochondrial activation

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The results of Western blotting confirmed that rapamycin (0.5 nM) significantly reduced phosphorylated S6 expression compared to untreated cells, and the combination of ASP (0.01 mg/mL) and rapamycin showed a more potent inhibitory effect on the expression of phosphorylation of S6 in both cells (Figure 6C)