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After filtration, centrifugation, precipitation, and resuspension, FLS were cultured in complete culture media (DMEM with 10% fetal bovine serum, 1% antibiotics) and maintained at 37 C in a humidified incubator of 5% CO 2 and 95% air

4a).These metabolites are involved in one-carbon metabolism, methylation processes, and amino acid biosynthesis, suggesting coordinated alterations in pathways relevant to cellular methylation and metabolic regulation in ASD

[DOI] [PMC free article] [PubMed] [Google Scholar] Pierce JP, et al

Tyrosine for mitigating stress and enhancing performance in healthy adult humans: A rapid evidence assessment of the literature