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After 4 weeks of observation, the tumor volume was recorded, and the tumors of the nude mice in each group were weighed

GCL knockdown was insufficient to trigger a significant increase in mitochondrial O 2 , but this was strongly potentiated by rotenone and rescued by mito GCL (mut

The experiments were performed at 25 C

Furthermore, kidneys from PKD1-knockout mice exhibit prominent features of ferroptosis, including the downregulation of SLC3A2, SLC7A11, and GPX4 and the upregulation of TFR1, divalent metal transporter 1, and HO-1, as well as mitochondrial abnormalities such as reduced mitochondrial cristae and ruptured mitochondrial outer membranes