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Primary antibodies, including CD206 antibody (Lot: YA798, MCE, USA) and iNOS antibody (Lot: HY-P80725, MCE, USA), were applied, followed by incubation with secondary antibodies, specifically goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated with Alexa Fluor 488 (Lot: A-11034, Thermo Fisher, USA)

investigated the regulatory effect of epithalon on the production of melatonin and cortisol in senescent monkeys, finding that the peptide significantly stimulated melatonin synthesis, thus normalizing the circadian rhythm of cortisol secretion [11]

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