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After washing once with RIPA buffer, three times with 1% (v/v) SDS, 8 M Urea in PBS, once with 1% (v/v) SDS in PBS and two times with PBS, pulled down proteins were either eluted by 2x Laemmli, including 100 M biotin, for western blot analysis or on-bead digested for mass spectrometry evaluation (see mass spectrometry methods)

Neural stem cell-derived EVs, on the other hand, deliver functional mitochondria to recipient cells, thereby modulating metabolic activity [51]

In the investigation of Lin et al., marine collagen peptides inhibited early alcoholic liver injury in female rats by improving oxidative stress and lipid metabolism ( The inflammatory response is another significant mechanism of heat stress and exercise fatigue-induced heatstroke (Figure 7)

Instead, intramuscular injections may be more suitable