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Incubation of these inhibitors with TGR in the absence of NADPH led to significantly attenuated enzyme inhibition compared to inhibition resulting from incubation in the presence of NADPH (Fig

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This extracellular and mislocalized intracellular copper exacerbates DCM through multiple pathways, activating the TGF-/Smad signaling pathway and amplifying oxidative stress, collectively promoting extracellular matrix deposition and fibrotic remodeling [171]