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Regular oil can be used, but we recommend preparing it without oil

59 It is the most significant mRNA in intestinal tissue, prompting the suggestion that it may have a role in intestinal first-pass metabolism, though others consider a physiological role, as opposed to a detoxification role also possible

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Assay for mitochondrial membrane potential (MMP) using JC-1 Determination of MMP was carried out using the fluorescent dye JC-1 [19] and analysed by laser confocal microscopy using LSM510META (Zeiss, Germany) and a dual-wavelength/double-beam recording microplate reader (Flex Station 384, Molecular Devices, USA)