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After removing the solvents from the polar phases using an SP Genevac EZ-2 series 4.0 centrifugal evaporator, the lyophilised extracts were resuspended in 500 L of a tissue-specific phosphate buffer containing 50 mM NaHPO, 1 mM 2,2,3,3-d-trimethylsilyl-propionic acid, sodium salt (TSP-d4), 50 L of D2O, and 2 mM sodium azide (NaN3) as an antibacterial agent