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The plate is placed on ice and cells are immediately treated with 35_ per well of ice cold lysis buffer, prepared according to the kit instructions (per assay plate, to 5ml of Tris lysis buffer add 100 of 50x protease inhibitor solution and 50 of each 100x phosphatase inhibitor solutions I and II)

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Made of 43 amino acids, TB-4 is found in just about every cell you have

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