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T4 has multiple biological activities that include downregulation of inflammatory chemokines and cytokines, and promotion of cell migration, blood vessel formation, cell survival, and stem cell maturation all contributing to the multiple wound healing properties observed in animal studies

Zhou N, Betancourt Cuellar SL, Gladish G, Antonoff MB , Hofstetter WL, Mehran RJ, Rice DV, Swisher SG, Walsh GL, Vaporciyan AA, Rajaram R

The most widely used cysteine detection method, Ellman's reagent (DTNB), reacts with virtually any free thiol in the sampleglutathione, coenzyme A, reduced protein sulfhydryls, even the cysteine residues exposed on the surface of albuminand reports their combined concentration as "cysteine." In a typical mammalian cell lysate, glutathione is present at millimolar concentrations, roughly 100- to 1,000-fold higher than free cysteine

However, the small sample size and in vitro granuloma model used thus far do not fully replicate the complex granulomatous environments observed in PTB patients