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109 used a brush functionalization technique to create a gradient of TGF-3, decreasing in concentration from the articulating surface to the subchondral region, and likewise created a reverse gradient of BMP-2

Malhi GS, Fritz K, Allwang C, Burston N, Cocks C, Harper M, et al

This axis regulates the gut microbiota and brain function bidirectionally through neural anatomical pathways, neuroimmune and neuroendocrine routes, the intestinal mucosal barrier, microbial metabolites, and the blood-brain barrier

burgdorferi lipoproteins were unsuccessful (Dunn et al., 1990), likely because of differences in the lipoprotein trafficking machinery