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Defining a PVN Glu DMV Ach small intestine circuit To identify the cellular and/or molecular mechanism(s) mediating the observed suppressive effect of morphine on small intestine-projecting DMV Ach neuronal activity, we conducted retrograde monosynaptic tracing by infusing an AAV2/Retro-hSyn-Cre virus into the small intestine, along with a modified rabies virus (EnvA-pseudotyped RV-G-EGFP) and Cre-dependent helper viruses (AAV-Ef1-DIO-TVA-mCherry and AAV-Ef1-DIO-RVG) into the DMV of C57 mice (Fig