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Western blot analysis Protein sample prepared in Laemmli loading buffer was loaded onto 8% polyacrylamide gel, TGx Mini-PROTEAN 4-15% precast gel (BioRad), or Bolt Bis-Tris Plus mini-Protein Gel 4-12% (Invitrogen) and run at 100-140 constant voltage in Tris-Glycine or MES buffer for their respective gels