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The reaction mixtures (1 g HepG2 nuclear protein, 1 pmol probes annealed on 95 C for 5 min in a heat block, 1 g poly d(I-C), 2 L of 5X Binding Buffer, 1.0 L of TF probe, 2.0 L of cold TF probe for competitive group, 1.5 L of anti-TCF4 antibody for supershift band) were incubated according to manufacturers protocol and then loaded on a 6% native polyacrylamide gel in 0.5% TBE buffer and blotted onto Biodyne B Nylon Membrane (0.45 m, Thermo Fisher Scientific, Gaithersburg, MD, USA)

The transcriptional signatures identified in GLP-1MK-801-treated animals were enriched for functional terms related to synaptic transmission, such as postsynaptic density and glutamatergic synapse (Fig