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Three male and three female rats were selected at each time point, and approximately 7 ml of whole blood was collected by heart puncture

We further characterized the organoids by imaging against a panel of hepatic markers for hepatocytes (HNF4 and ASGR1), macrophages (Kupffer cells) (CD68), cholangiocytes (CK7), hepatic stellate cells (SMA) and vasculature (CD31), where all markers were represented (Supplementary Fig