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doi: 10.1093/femsre/fuaa039 255 SrirengarajV.RazafindralamboH

Following incubation and blocking with phosphate buffered saline (PBS) containing 0.1% Triton and 0.5% bovine serum albumin (BSA), each coverslip was incubated with primary antibodies against Nrf2 (Santa Cruz rabbit polyclonal IgG, 2.5 g/mL) for 1 h at room temperature, followed by incubation with secondary fluorophore-conjugated antibodies (Alexa Fluor 488 (green) donkey anti-rabbit IgG, Invitrogen, 10 g/mL) for another 1 h at room temperature

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The outflow facility could be increased by cyclic adenosine monophosphate (cAMP) administration in anterior chamber for the maintenance of IOP 34