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For GUV experiments, raw rim binding of each domain was normalized by the fluorescence signal of a 1 M protein solution from the same purification batch to reduce variabilities in fluorescence from different purification batches

The polyol pathway works as the most important source of ROS generation in the neuron, retina, and nephron, and sorbitol increase concentration has been associated with neuropathy, retinopathy, and nephropathy of DM (Figure 4) (Eckers et al., 2012

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Trends Neurosci (1985) 8:7983