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This vitamin, known as thiamine, is active in its thiamine diphosphate (TDP) form, which is a cofactor of cytosolic transketolase and three mitochondrial enzymes involved in energy production, namely mitochondrial pyruvate dehydrogenase (PDH) [51], -ketoglutarate dehydrogenase (-KGDH) [52], and branched-chain -keto-acid dehydrogenase (BCKDH) [53] (Figure 1)

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Step 2 Membrane Binding and Insertion: Upon binding to membrane-localised HDM-2, PNC-27 inserts into the lipid bilayer via its C-terminal transmembrane-penetrating domain, anchoring the PNC-27HDM-2 complex within the cell membrane