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Fred Schaufele, XCellAssay Research Summary: This project aimed at developing a cell-based nuclear receptor translocation bioassay has largely achieved the projected goals

For WB, 30 g of proteins from tissue and cell lysates were mixed with 8 L of sample buffer (Tris 0.5% (pH 6.8), glycerol 20%, sodium dodecylsulfate (SDS) 10%, 2--mercaptoethanol, bromophenol blue 0.1%), and RIPA buffer to a total sample volume of 32 L

Samples containing 20 g of protein were run on an SDS-PAGE gel and transferred to polyvinyl difluoride (PVDF) membranes (Immobilon-F, Millipore) using a transfer tank (TE22, Hoefer)

This approach may help reduce common reactions like bloating, flushing, headaches, and nasal congestion after eating