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These antigens have been demonstrated to elicit immune responses in individuals with late-stage colorectal adenocarcinomas, as well as in in those with advanced adenomas, making them potential targets for vaccine prevention in individuals who are at high risk for cancer development, such as those with LS [132136]

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The next day, sections were incubated with secondary antibody in 1 PBS containing 1% BSA and 0.3% Triton X-100 for 2 h at room temperature