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Common reactions include the formation of thiocarbamates with cysteine residues in proteins and glutathione (GSH), as well as alkylation reactions with -amino groups in N-terminal protein residues, -amino groups of lysine, and secondary amines like proline

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Intranasal delivery represents the most significant methodological advancement because it bypasses hepatic first-pass metabolism entirely, but technique sensitivity (nasal cavity anatomy, administration volume, head positioning post-dose) introduces reproducibility challenges that subcutaneous injection avoids