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EVs were aliquoted and stored in low-protein-binding tubes with 25 mM D(+)-trehalose anhydrous (Chem-Impex International, Wood Dale, IL, USA) at 80 C to be used within 2 weeks

With advancing age, cellular NAD levels decline significantly through three main mechanisms: reduced synthesis (decreased NAMPT), increased catabolism (CD38 hyperactivation under inflammaging conditions) and excessive consumption by DNA repair systems. Intravenous administration of NAD or its precursors such as NR (nicotinamide riboside) and NMN (nicotinamide mononucleotide) aims to restore a more youthful biosynthetic and reparative capacity to ageing cells

Since UA-lowering therapies induce sustainable reduction in UA levels, it was expected that interventional studies in analogy with the Mendelian randomization studies, will help in clarification of the causal relationship between UA and CVD