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In addition, the expression of tight junction proteins (ZO-1, Occludin, Claudin1) was significantly reduced by LY294002 administration, consequently inhibiting the improvement effect of HT, as confirmed by the disappearance of the improvement in Claudin1 immunofluorescence (Fig

J Exp Clin Cancer Res 36:19

These included, among others, the difficulty of sectional sampling for fluids as well as the cells for spatial characterization of the patterned tissue responses