Astrocytes could still survive in culture several days after exposure, underlining the importance of glycolysis for maintaining their function and neuronal dependence from undisturbed astrocytic metabolism (90)
Then the membrane was incubated with the primary antibodies at 4C overnight, using the following dilutions: rabbit anti-ORP5 (SIGMA, 1:1000), rabbit anti-ORP8 (Gentex, 1:1000), mouse anti-Cytochrome C (BD Pharmingen, 1:2000), mouse anti-PDI (GeneTex, 1:500), rabbit anti-PLN3 (SIGMA, 1:800), mouse anti-IP3R-3 (BD Transduction Laboratories, 1:2000), mouse anti-DRP1 (BD Transduction Laboratories, 1:1000), rabbit anti-MFF (Proteintech1:1000), rabbit anti-MFN1 (Proteintech, 1:1000), rabbit anti-MFN2 (Proteintech, 1:1000), mouse anti-OPA-1 (BD Transduction Laboratories, 1:1000), mouse anti-GAPDH (GeneTex, 1:10000) in 5%milk in TBST
Besides, icariin inhibits osteoclast generation and significantly suppresses the bone metastasis of prostate cancer (RM-1) cells [704]
it can elicit hypersensitivity and systemic allergic reactions, and its short half-life requires treatment via a slow injection over a period of 4-12 hours (Heli 2011)
