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Immunotherapy is powerful, but it can be very hard on the body

Estos pacientes necesitan cuidar muy bien el msculo cardiaco, que est compuesto por clulas denominadas cardiocitos, estos trabajan incesantemente contrayendo las fibras musculares del corazn para lograr bombear la sangre hasta los lugares ms alejados y as oxigenar a todo el cuerpo

For Immunofluorescence stainings, the tissue sections were fixed with 4% paraformaldehyde (PFA) for 10 min and permeabilized using 0.1% Triton X-100 for 10 min at room temperature, followed by blocking using 3% bovine serum albumin (BSA) for 1 h at room temperature

C/EBP knockdown, for instance, decreased expression of all genes measured, including ATF4, which was also reflected in loss of ATF4 protein (Figure 2C-D)