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Half of the sample was used for this protocol and half were pelleted and snap-frozen for nuclear extraction described below

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For example, cuprizone administration reduces the mitochondrial enzymes monoamine oxidase (as early as 3 days [27]) and cyt c oxidase [28, 29] in the brain of mice, both of which require copper for catalysis [126]

These differences may be explained by numerous factors, such as variable disease prevalence in select populations