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Then, 3.5 l of the sample was applied onto 45 s glow-discharged quantifoil grids and vitrified in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) at 100% humidity, 7 s blotting time and 10 s waiting time

Notably, in an experiment with a high-fructose (60%, 8 weeks) diet, although the use of LA did not reduce TG levels, LA reversed mild portal endotoxemiainduced inflammation

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Results of the statistical tests were corrected for multiple testing using the Benjamini-Hochberg method