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The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)

In patients with HS, splenectomy is the gold standard therapeutic option for severe hematologic phenotype, but indications and long-term cost-effectiveness in moderate to mild HS are not well established (51)