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GHK-Cu is fully endogenous, present in human plasma, saliva, and urine, and released at sites of tissue injury through breakdown of SPARC and type I collagen

The mice were acclimated for one week before the experiment, during which their health status was monitored

Hypotheses 143:109862

First, a bacterial reverse mutation test (Ames test) assessed AOD9604s mutagenic potential using four histidine-dependent auxotrophic mutants of Salmonella typhimurium (TA1535, TA1537, TA98, TA100) and one tryptophan-dependent auxotrophic mutant of Escherichia coli (WP2 uvrA), using both plate incorporation and pre-incubation methods at doses of 0, 1.6, 8, 40, 200, 1,000, and 2,000 g AOD9604/plate