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There may also be some pressure at the insertion site during the procedure

This protective response might be an attempt to reduce the levels of oxidative stress but alternatively might accelerate ferroptosis in case of excessive HMOX-1 activation [30]

To prepare hippocampal slices, horizontal sections (400 m thick) at the middle septotemporal levels were cut in ice-cold cutting solution using a Leica VT1000S vibratome (Leica Microsystems) and allowed to recover at 32 C for at least 1 h in an oxygenated (95% O 2 /5% CO 2 ) artificial cerebrospinal fluid (aCSF) solution (124 mM NaCl, 2 mM KCl, 2 mM MgSO 4 , 2 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 10 mM Glucose, with pH 7.3 and osmolarity 300 mOsm/L)