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6.1 Regulation of reproductive-related hormones The HPO axis is a highly coordinated neuroendocrine system integrating the hypothalamus, pituitary gland, and ovaries to regulate reproductive cyclicity

The 24-h UTP, serum scr and serum BUN concentrations of mice were detected with the specified detection kit

Glutathione is a natural anti-oxidant made from amino acids like glycine, cysteine and glutamic acids present in the Human body

Cell culture and transfection/electroporation 293 T human embryonic kidney cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 units/mL of penicillin, and 100 g/mL of streptomycin and then transfected with expression vectors via the calcium phosphate-DNA precipitation method 33