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Under normal conditions there is always a small amount of inactive apoenzyme in tissues, although most is present as the catalytically active holoenzyme, with pyridoxal phosphate bound

pyroglutamic (5-oxoproline) acidosis pyroglutamic (5-oxoproline) acidosis pathophysiology pathogenesis of pyroglutamic acidosis The primary driver may be cysteine deficiency : Cysteine deficiency causes glutamylcysteine to be shunted into 5-oxoproline

The chronic exposure of rats to non-labelled aspartame at 200 mg/kg for 10 days before administration of the radiolabelled dose resulted in increased accumulation of radiolabel, suggesting that there may be a cumulative increase in the number of formaldehyde adducts derived from aspartame in tissue proteins and nucleic acids (Trocho et al., 1998)

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