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Membranes were washed 3 times for 5 minutes each the next day in high-salt TBST (20 mM Tris pH 7.6, 500 mM NaCl, 0.05% Tween-20, 0.01% sodium azide) and then incubated for 1 hour at room temperature, shaking, with 800CW goat anti-rabbit IgG secondary antibody (Li-Cor Biosciences, 926-32211) and 680RD goat anti-mouse IgG secondary antibody (Li-Cor Biosciences, 926-68070) diluted 1:10,000 in high-salt TBST

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T1 signal of intravenously injected AuNWs is in OFF state which can switch to ON in the presence of high GSH concentration in tumor cells [78]

Results: A total of 424 eligible patients were included, with 31.1% of DN patients and 13.2% of DR patients