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Description

The PCR reaction is carried out in a 50 microliter volume containing 1PCR buffer, 2 mM of each dNTP, 0.7 units of High Fidelity Taq polymerse, 5 primer mix, 3 primer mix and 7.5 microliters of cDNA

This stimulus amplitude fluctuated between 0.5 mA (Jongkees et al., 2018) and 12 mA (Trevizol et al., 2016), similar to the range reported in other literature